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The overarching aim of the project is to re-programme the lifestyle of Pseudomonas putida and designing a modular, streamlined bacterial platform for bespoke biocatalysis. Steps that lead to this overall aim are:

Step 1 - Generation of strains with a genome streamlined chassis still enabling growth and production but freed of non-essential functionalities and re-factored for optimal functioning.

Step 2 - Development of novel ATP sensoring and control loops.

Step 3 - Design and implementation of strains uncoupling growth from production of specialty and bulk chemicals.

Step 4 - Harness of non-native butanol and isobutanol production in re-factored chassis.

Step 5 - Opening the door for novel bio-sustainable routes by accessing butene and butadiene as well as a specialty chemical with potential use in crop protection from glucose.

Step 6 - Validation of the value and strength of the refactored Pseudomonas putida–based platform for the production of the bulk and specialty chemicals indicated above.

Step 7 - Translation of the knowledge and performance testing for potential end use applications.

This novel production platform shall comprise:

  • a streamlined genome still enabling growth but liberated from non-essential functionalities and, presumably, more amenable for process control
  • novel plug-in loci under the control of orthogonal regulation systems (small RNAs)
  • novel ATP sensoring and control loops decoupling ATP availability from growth, and
  • new pathways to produce ATP-demanding (iso-)butanol and derivatives as building blocks for chemicals and biofuels, as an example of a class of specialty chemical of high agricultural and industrial importance

 

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This project has received funding from the European Union’s Horizon 2020

research and innovation programme under grant agreement No 635536.

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