In their experimental work with Pseudomonas putida the EmPowerPutida partners have developed SOPs on genome engineering (e.g. MAGE, error-prone PCR), modification of bacterial growth and quantification of the products of enzymatic reactions as well as molecular biology techniques (e.g. RNA extraction, Northern blotting, PCR).

The SOPs all use a standard template to describe the purpose of the experiment, equipment and bacterial strains, media, procedure and any troubleshooting or biosafety issues.

EmPowerPutida is collaborating with IBISBA sharing ideas on key elements to include in experimental SOPs to help inform how SOPs can be improved.

The final SOPs are accessible in the categories (Molecular Biology, Microbiology, Cultivation and Production, Analytics) through the following links: 

Molecular Biology

 

Cultivation and Production

 

Microbiology

 

Analytics

 

 

 

 

 

 

 

 

 

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This project has received funding from the European Union’s Horizon 2020

research and innovation programme under grant agreement No 635536.

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