In their experimental work with Pseudomonas putida the EmPowerPutida partners have developed SOPs on genome engineering (e.g. MAGE, error-prone PCR), modification of bacterial growth and quantification of the products of enzymatic reactions as well as molecular biology techniques (e.g. RNA extraction, Northern blotting, PCR).
The SOPs all use a standard template to describe the purpose of the experiment, equipment and bacterial strains, media, procedure and any troubleshooting or biosafety issues.
These SOPs are available at the following links: