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In their experimental work with Pseudomonas putida the EmPowerPutida partners have developed SOPs on genome engineering (e.g. MAGE, error-prone PCR), modification of bacterial growth and quantification of the products of enzymatic reactions as well as molecular biology techniques (e.g. RNA extraction, Northern blotting, PCR).

The SOPs all use a standard template to describe the purpose of the experiment, equipment and bacterial strains, media, procedure and any troubleshooting or biosafety issues.

These SOPs are available at the following links:

Bradford Protein Assay

Evaluation of kinase type enzymes via ADP-dependent ATP depletion assay via NADH-dependent enzyme cascade

Extraction of total RNA from P. putida

Oxygen gradients for adaptation of bacteria to varying oxygen availability

Performing a batch and chemostat cultivation with P. putida in labscale

Polyacrylamide Northern Blot

Preparation and transformation of electrocompetent bacterial cells

Preparation of gene libraries by error-prone PCR

Shaking Flask cultures of Pseudomonas putida

Two-phase based whole cell system for bioconversions

 

 

 

 

 

 

 

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This project has received funding from the European Union’s Horizon 2020

research and innovation programme under grant agreement No 635536.

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